Product Name: PKR (phospho Thr258) Polyclonal Antibody
Catalog No.: ALP0796
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: EIF2AK2
Protein Name: Interferon-induced double-stranded RNA-activated protein kinase
Human Gene ID: 5610
Human Swiss Prot No.: P19525
Other Name: EIF2AK2; PKR; PRKR; Interferon-induced; double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein k
Subcellular Location: Cytoplasm . Nucleus . Cytoplasm, perinuclear region . Nuclear localization is elevated in acute leukemia, myelodysplastic syndrome (MDS), melanoma, breast, colon, prostate and lung cancer patient samples or cell lines as well as neurocytes from advanced Creutzfeldt-Jakob disease patients. .
Expression: Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas, and which correlates with tumor progression and invasiveness or risk of progression.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKR (Phospho-Thr258) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKR (Phospho-Thr258) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKR (Phospho-Thr258) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.