Product Name: PKC θ (phospho Ser695) Polyclonal Antibody
Catalog No.: ALP0719
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: PRKCQ
Protein Name: Protein kinase C theta type
Human Gene ID: 5588
Human Swiss Prot No.: Q04759
Other Name: PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta
Subcellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Expression: Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC thet (Phospho-Ser695) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser695) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15′, using PKC thet (Phospho-Ser695) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.