PKC ζ (phospho Thr560) Polyclonal Antibody

Catalog No.: 

Product Name: PKC ζ (phospho Thr560) Polyclonal Antibody

Catalog No.: ALP0230

Reactivity: Human;Mouse;Rat;Monkey

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: PRKCZ

Protein Name: Protein kinase C zeta type

Human Gene ID: 5590

Human Swiss Prot No.: Q05513

Other Name: PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

Subcellular Location: Cytoplasm . Endosome . Cell junction . Membrane ; Peripheral membrane protein . In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes (PubMed:9566925). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083). Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651). Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity). .; [Isoform 2]: Cytoplasm .

Expression: Expressed in brain, and to a lesser extent in lung, kidney and testis.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30′, using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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