Product Name: PKC ε (phospho Ser729) Polyclonal Antibody
Catalog No.: ALP0340
Reactivity: Human;Mouse;Rat
Applications: WB;ELISA;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Storage Stability: -20°C/1 year
Gene Name: PRKCE
Protein Name: Protein kinase C epsilon type
Human Gene ID: 5581
Human Swiss Prot No.: Q02156
Other Name: PRKCE; PKCE; Protein kinase C epsilon type; nPKC-epsilon
Subcellular Location: Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane . Cytoplasm, perinuclear region . Nucleus . Translocated to plasma membrane in epithelial cells stimulated by HGF (PubMed:17603037). Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA-treated cells (By similarity). .
Expression: Expressed in cumulus cells (at protein level).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC epsilon (Phospho-Ser729) Antibody
Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30′, using PKC epsilon (Phospho-Ser729) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.