PKAα/β/γ cat (phospho Thr197) Polyclonal Antibody

Catalog No.: 

Product Name: PKAα/β/γ cat (phospho Thr197) Polyclonal Antibody

Catalog No.: ALP0226

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF/ICC;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: PRKACA/PRKACB

Protein Name: cAMP-dependent protein kinase catalytic subunit alpha/beta

Human Gene ID: 5566/5567

Human Swiss Prot No.: P17612/P22694/P22612

Other Name: PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma

Subcellular Location: Cytoplasm. Cell membrane. Nucleus . Mitochondrion . Membrane ; Lipid-anchor . Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (PubMed:21085490). .; [Isoform 2]: Cell projection, cilium, flagellum . Cytoplasmic vesicle, secretory vesicle, acrosome . Expressed in the midpiece region of the sperm flagellum (PubMed:10906071). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). .

Expression: Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKA CAT (Phospho-Thr197) Antibody


Immunofluorescence analysis of A549 cells, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.


Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.


Western blot analysis of lysates from mouse brain, using PKA CAT (Phospho-Thr197) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

Download SDS – English
Download SDS – Norwegian

Leave a Reply

Your email address will not be published. Required fields are marked *