PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody

Catalog No.: 

Product Name: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody

Catalog No.: ALP0224

Reactivity: Human;Mouse;Rat;Monkey

Applications: WB;IHC-p;IF/ICC;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: PIK3R1/PIK3R3

Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha/gamma

Human Gene ID: 5295

Human Swiss Prot No.: P27986/Q92569

Other Name: PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

Subcellular Location: nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,

Expression: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody


Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.


Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30′, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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