Product Name: PI 3 Kinase Class 3 Polyclonal Antibody
Catalog No.: ALT5759
Reactivity: Human;Mouse;Rat;Predicted:Cow:PIG
Applications: WB;IHC-p
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000;IHC-p 1:50-300
Storage Stability: -20°C/1 year
Gene Name: PIK3C3 VPS34
Protein Name: PI 3 Kinase Class 3
Human Gene ID: 5289
Human Swiss Prot No.: Q8NEB9
Other Name: phosphoinositide-3-kinase, class 3
Subcellular Location: Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (PubMed:14617358). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .
Expression: Ubiquitously expressed, with a highest expression in skeletal muscle.
Western Blot analysis of 293T 3T3 cells using PI 3 Kinase Class 3 Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.