Product Name: Per2 (phospho Ser662) Polyclonal Antibody
Catalog No.: ALP0764
Reactivity: Human;Mouse
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: PER2
Protein Name: Period circadian protein homolog 2
Human Gene ID: 8864
Human Swiss Prot No.: O15055
Other Name: PER2; KIAA0347; Period circadian protein homolog 2; hPER2; Circadian clock protein PERIOD 2
Subcellular Location: [Isoform 1]: Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Translocate to the nucleus after phosphorylation by CSNK1D or CSNK1E. Also translocated to the nucleus by CRY1 or CRY2. PML regulates its nuclear localization. .; [Isoform 2]: Nucleus, nucleolus .
Expression: Widely expressed. Found in heart, brain, placenta, lung, liver, skeleatal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low levels in lung. Isoform 2 is expressed in keratinocytes (at protein level).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Period Circadian Protein 2 (Phospho-Ser662) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30′, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.