PDPN rabbit pAb

Catalog No.: 

Product Name: PDPN rabbit pAb

Catalog No.: ALT6610

Reactivity: Human; Mouse;Rat

Applications: WB;ELISA;IHC-p

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

Storage Stability: -20°C/1 year

Gene Name: PDPN GP36 PSEC0003 PSEC0025

Protein Name: PDPN

Human Gene ID: 10630

Human Swiss Prot No.: Q86YL7

Other Name:

Subcellular Location: [Podoplanin]: Membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane ; Single-pass type I membrane protein . Cell projection, filopodium membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Membrane raft . Apical cell membrane . Basolateral cell membrane . Cell projection, invadopodium . Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles (By similarity). Association to the lipid rafts is required for PDPN-induced epithelial to mesenchymal transition (EMT) (PubMed:21376833). Colocalizes with CD9 in tetraspanin microdomains (PubMed:18541721). Localized at invadopodium adhesion rings in tumor cell. Association to the lipid rafts is essential for PDPN recruitment to invadopodia and ECM degradation (PubMed:25486435). .; [29kDa cytosolic podoplanin intracellular domain]: Cytoplasm, cytosol .

Expression: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of eaYy oral squamous cell carcinomas.


Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night


Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

For research use only. Not for use in diagnostic procedures.

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