Product Name: PAKα/β/γ (phospho Ser144/141/139) Polyclonal Antibody
Catalog No.: ALP0667
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: PAK1/PAK2/PAK3
Protein Name: Serine/threonine-protein kinase PAK 1/Serine/threonine-protein kinase PAK 2/Serine/threonine-protein kinase PAK 3
Human Gene ID: 5058/5062/5063
Human Swiss Prot No.: Q13153/Q13177/O75914
Other Name: PAK1; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK2; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK3; OPHN3; Serine/threonine-p
Subcellular Location: Cytoplasm . Cell junction, focal adhesion . Cell projection, lamellipodium . Cell membrane . Cell projection, ruffle membrane . Cell projection, invadopodium . Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with MORC2 on damaged chromatin (PubMed:23260667). Localization to the centrosome does not depend upon the presence of gamma-tubulin (PubMed:27012601). Localization of the active, but not inactive, protein to the adhesions and edge of lamellipodia is mediated by interaction with GIT1 (PubMed:11896197). .
Expression: Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PAK1/2/3 (Phospho-Ser144/141/139) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using PAK1/2/3 (Phospho-Ser144/141/139) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from mouse brain, using PAK1/2/3 (Phospho-Ser144/141/139) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.