Product Name: Ob-R Polyclonal Antibody
Catalog No.: ALT5282
Reactivity: Human;Rat;Mouse;
Applications: WB;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: LEPR OBR
Protein Name: Leptin receptor
Human Gene ID: 3953
Human Swiss Prot No.: P48357
Other Name: LEPR; DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295
Subcellular Location: Cell membrane ; Single-pass type I membrane protein . Basolateral cell membrane .; [Isoform E]: Secreted .
Expression: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218). Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075).
Western Blot analysis of HeLa, K562, MCF7 cells using Ob-R Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
For research use only. Not for use in diagnostic procedures.