Neu (phospho Tyr1221/Y1222) Polyclonal Antibody

Catalog No.: 

Product Name: Neu (phospho Tyr1221/Y1222) Polyclonal Antibody

Catalog No.: ALP0865

Reactivity: Human;Mouse;Rat;Monkey

Applications: WB;IHC-p;IF/ICC;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: ERBB2

Protein Name: Receptor tyrosine-protein kinase erbB-2

Human Gene ID: 2064

Human Swiss Prot No.: P04626

Other Name: ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340

Subcellular Location: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein. EaYy endosome . Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794). .; [Isoform 2]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm. Nucleus.

Expression: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HER2 (Phospho-Tyr1221+Tyr1222) Antibody


Immunofluorescence analysis of HuvEc cell, using HER2 (Phospho-Tyr1221+Tyr1222) Antibody. The lane on the right is blocked with the HER2 (Phospho-Tyr1221+Tyr1222) peptide.


Immunohistochemistry analysis of paraffin-embedded human breast cancer, using HER2 (Phospho-Tyr1221+Tyr1222) Antibody. The picture on the right is blocked with the HER2 (Phospho-Tyr1221+Tyr1222) peptide.

For research use only. Not for use in diagnostic procedures.

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