Product Name: mTOR (phospho Ser2481) Polyclonal Antibody
Catalog No.: ALP1134
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. ,WB 1:500-2000
Storage Stability: -20°C/1 year
Gene Name: MTOR
Protein Name: Serine/threonine-protein kinase mTOR
Human Gene ID: 2475
Human Swiss Prot No.: P42345
Other Name: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar
Subcellular Location: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). .
Expression: Expressed in numerous tissues, with highest levels in testis.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using mTOR (Phospho-Ser2481) Antibody
Immunofluorescence analysis of NIH/3T3 cells, using mTOR (Phospho-Ser2481) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-Ser2481) Antibody. The picture on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.