Product Name: M-CSF Polyclonal Antibody

Catalog No.: ALT5553

Reactivity: Human;Rat;Mouse;

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: CSF1

Protein Name: Macrophage colony-stimulating factor 1

Human Gene ID: 1435

Human Swiss Prot No.: P09603

Other Name: CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim

Subcellular Location: Cell membrane ; Single-pass type I membrane protein .; [Processed macrophage colony-stimulating factor 1]: Secreted, extracellular space.

Expression: Endometrium,Kidney,Pancreatic carcinoma,T lymphoblast,Trophoblast,Urine,

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western Blot analysis of 3T3 cells using M-CSF Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100

Western Blot analysis of NIH-3T3, Hela cells using M-CSF Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

For research use only. Not for use in diagnostic procedures.

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