Lfc (phospho Ser885) Polyclonal Antibody

Catalog No.: 

Product Name: Lfc (phospho Ser885) Polyclonal Antibody

Catalog No.: ALP0398

Reactivity: Human;Mouse;Rat

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: ARHGEF2

Protein Name: Rho guanine nucleotide exchange factor 2

Human Gene ID: 9181

Human Swiss Prot No.: Q92974

Other Name: ARHGEF2; KIAA0651; LFP40; Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40

Subcellular Location: Cytoplasm, cytoskeleton . Cytoplasm . Cell junction, tight junction . Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Cell projection, ruffle membrane . Cytoplasmic vesicle . Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085). Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560). Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730). .

Expression: Brain,Cervix carcinoma,Epithelium,Platelet,


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody


Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30′, using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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