Keratin-pan Polyclonal Antibody

Catalog No.: 

Product Name: Keratin-pan Polyclonal Antibody

Catalog No.: ALT5870

Reactivity: Human;Mouse;Rat

Applications: WB;ELISA;IHC-p

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

Storage Stability: -20°C/1 year

Gene Name: Keratin

Protein Name: Keratin

Human Gene ID: 3849

Human Swiss Prot No.: P35908/Q01546/P12035/P13647/P02538/P04259/P48668/Q3SY84/Q14CN4/Q86Y46/Q7RTS7/O95678/Q5XKE5/P08729/P05787/Q9NSB2

Other Name:

Subcellular Location: extracellular space,nucleus,cytoplasm,Golgi apparatus,intermediate filament,membrane,keratin filament,intermediate filament cytoskeleton,extracellular exosome,

Expression: Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization.

Western blot analysis of mouse-lung mouse-brain mouse-kidney mouse-heart lysate, antibody was diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

For research use only. Not for use in diagnostic procedures.

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