Product Name: Iba1 Polyclonal Antibody
Catalog No.: ALN2165
Reactivity: Human;Rat;Mouse
Applications: WB;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: AIF1 G1 IBA1
Protein Name: Allograft inflammatory factor 1 (AIF-1) (Ionized calcium-binding adapter molecule 1) (Protein G1)
Human Gene ID: 199
Human Swiss Prot No.: P55008
Other Name:
Subcellular Location: Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, phagocytic cup . Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. .
Expression: Detected in T-lymphocytes and peripheral blood mononuclear cells.
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
For research use only. Not for use in diagnostic procedures.