Product Name: HSP90β (phospho Ser254) Polyclonal Antibody
Catalog No.: ALP0857
Reactivity: Human;Mouse;Rat;Monkey
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: HSP90AB1
Protein Name: Heat shock protein HSP 90-beta
Human Gene ID: 3326
Human Swiss Prot No.: P08238
Other Name: HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84
Subcellular Location: Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673). Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762). .
Expression: Amygdala,Brain cortex,Colon,Colon carcinoma,Embryon
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP90B (Phospho-Ser254) Antibody
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15′, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30′, using HSP90B (Phospho-Ser254) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.