Product Name: HMG-1 (Acetyl Lys12) Polyclonal Antibody
Catalog No.: ALK0060
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: HMGB1
Protein Name: High mobility group protein B1
Human Gene ID: 3146
Human Swiss Prot No.: P09429
Other Name: HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1
Subcellular Location: Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). An increased chromatin association is observed when associated with the adenovirus protein pVII (PubMed:27362237). .
Expression: Ubiquitous. Expressed in platelets (PubMed:11154118).
Western Blot analysis of HepG2 cells treated with UV using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
For research use only. Not for use in diagnostic procedures.