Product Name: Histone H4 (Acetyl Lys16) Polyclonal Antibody
Catalog No.: ALK0187
Reactivity: Human;Mouse;Rat
Applications: IF/ICC;WB;IHC-p;
Source: Polyclonal, Rabbit,IgG
Formulation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration:
Dilution: IF: 1:50-200 WB: 1:1000-2000 IHC 1:50-300
Storage Stability: -20°C/1 year
Gene Name: HIST1H4A
Protein Name: Histone H4
Human Gene ID: 121504
Human Swiss Prot No.: P62805
Other Name: HIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FE; HIST1H4K; H4/D; H4FD; HIST1H4L; H4/K; H4FK; HIST2H4A; H4/N; H4F2; H4FN; HIST2H4; HIST2H4B; H4/O; H4FO; HIST4H4; Histone H4;H4k16AC; YM3317
Subcellular Location: Nucleus. Chromosome.
Expression: B-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call
if-human-lung39.jpg)
Immunofluorescence analysis of human-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
if-rat-lung43.jpg)
Immunofluorescence analysis of rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
poly-ihc-human-uterus.jpg)
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
poly-ihc-rat-lung.jpg)
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
poly-ihc-mouse-heart.jpg)
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
For research use only. Not for use in diagnostic procedures.