GSDMD n-ternal rabbit pAb

Catalog No.: 

Product Name: GSDMD n-ternal rabbit pAb

Catalog No.: ALT7991

Reactivity: Human;Mouse;Rat

Applications: IF/ICC;WB;IHC-p

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300

Storage Stability: -20°C/1 year

Gene Name: GSDMD

Protein Name: Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN

Human Gene ID:

Human Swiss Prot No.: P57764

Other Name:

Subcellular Location: [Gasdermin-D]: Cytoplasm, cytosol . Inflammasome . In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. .; [Gasdermin-D, N-terminal]: Cell membrane ; Multi-pass membrane protein . Secreted . Released in the extracellular milieu following pyroptosis. .; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol .

Expression: Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.

Immunofluorescence analysis of un-treated (left) A549 and UV treated(right) A549 cell. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

Western blot analysis of lysates from 1)mouse-brain, 2)Hela cells treated by UV 15min,3) HepG2 cells, primary antibody was diluted at 1:1000, 4°over night, secondary antibody HRP goat anti rabbit (Immunoway:RS0002) was diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded human Moderately differentiated hepatocellular carcinoma Antibody was diluted at 1:200(4° overnight).

For research use only. Not for use in diagnostic procedures.

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