Product Name: GM130 Polyclonal Antibody
Catalog No.: ALT6291
Reactivity: Human; Mouse; Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: GOLGA2
Protein Name: GM130
Human Gene ID: 2801
Human Swiss Prot No.: Q08379
Other Name: Golgin subfamily A member 2 (130 kDa cis-Golgi matrix protein;GM130;GM130 autoantigen;Golgin-95)
Subcellular Location: Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein ; Cytoplasmic side . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton, spindle pole . Associates with the mitotic spindle during mitosis (PubMed:26165940). .
Expression: Brain,Cervix,Colon adenocarcinoma,Epithelium,Heart,Hepatocyte,Liver,Placenta,Thymus,
Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.
Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
For research use only. Not for use in diagnostic procedures.