Product Name: GLI-3 Polyclonal Antibody
Catalog No.: ALT1914
Reactivity: Human;Mouse;Rat
Applications: IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: GLI3
Protein Name: Transcriptional activator GLI3
Human Gene ID: 2737
Human Swiss Prot No.: P10071
Other Name: GLI3; Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3-190; GLI3 full length protein; GLI3FL
Subcellular Location: Nucleus. Cytoplasm. Cell projection, cilium. GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1.
Expression: Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.