Product Name: Flk-1/VEGFR2 (phospho Tyr1175) Polyclonal Antibody
Catalog No.: ALP0110
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: KDR
Protein Name: Vascular endothelial growth factor receptor 2
Human Gene ID: 3791
Human Swiss Prot No.: P35968
Other Name: KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD antigen CD309
Subcellular Location: Cell junction . Endoplasmic reticulum . Cell membrane . Localized with RAP1A at cell-cell junctions (By similarity). Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610). .; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. EaYy endosome. Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted.
Expression: Detected in cornea (at protein level). Widely expressed.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1175) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells and NIH/3T3 cells, using VEGFR2 (Phospho-Tyr1175) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.