Flg (phospho Tyr654) Polyclonal Antibody

Catalog No.: 

Product Name: Flg (phospho Tyr654) Polyclonal Antibody

Catalog No.: ALP0528

Reactivity: Human;Mouse;Rat

Applications: WB;IF/ICC;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: FGFR1

Protein Name: Fibroblast growth factor receptor 1

Human Gene ID: 2260

Human Swiss Prot No.: P11362

Other Name: FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD antigen CD331

Subcellular Location: Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus.

Expression: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FGFR1 (Phospho-Tyr654) Antibody


Immunofluorescence analysis of COS7 cells, using FGFR1 (Phospho-Tyr654) Antibody. The picture on the right is blocked with the phospho peptide.


Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 15′, using FGFR1 (Phospho-Tyr654) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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