Product Name: FANCD2 Polyclonal Antibody
Catalog No.: ALT1675
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: FANCD2
Protein Name: Fanconi anemia group D2 protein
Human Gene ID: 2177
Human Swiss Prot No.: Q9BXW9
Other Name: FANCD2; FACD; Fanconi anemia group D2 protein; Protein FACD2
Subcellular Location: Nucleus . Concentrates in nuclear foci during S phase and upon genotoxic stress. At the onset of mitosis, excluded from chromosomes and diffuses into the cytoplasm, returning to the nucleus at the end of cell division. Observed in a few spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites and could be sites of replication fork stalling. The foci are frequently inteYinked through BLM-associated ultra-fine DNA bridges. Following aphidicolin treatment, targets chromatid gaps and breaks.
Expression: Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FANCD2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HT-29 cells, treated with Calyculin A 50ng/ml 30′, using FANCD2 Antibody. The lane on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.