Ezrin (phospho Thr566) Polyclonal Antibody

Catalog No.: 

Product Name: Ezrin (phospho Thr566) Polyclonal Antibody

Catalog No.: ALP0106

Reactivity: Human;Mouse;Rat

Applications: WB;ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: EZR

Protein Name: Ezrin

Human Gene ID: 7430

Human Swiss Prot No.: P15311

Other Name: EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

Subcellular Location: Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Cell projection, microvillus . Localization to the apical membrane of parietal cells depends on the interaction with PALS1. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side). .

Expression: Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ezrin (Phospho-Thr566) Antibody

Western blot analysis of lysates from Jurkat cells treated PMA 125ng/ml 30′, using Ezrin (Phospho-Thr566) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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