Product Name: ERK 2 Polyclonal Antibody
Catalog No.: ALT1627
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: MAPK1
Protein Name: Mitogen-activated protein kinase 1
Human Gene ID: 5594
Human Swiss Prot No.: P28482
Other Name: MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2
Subcellular Location: Cytoplasm, cytoskeleton, spindle . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm . Membrane, caveola . Cell junction, focal adhesion . Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization. .
Expression: Brain,Epithelium,Lung,Platelet,T-cell,
Western blot analysis of lysates from Jurkat and 293 cells, using p42 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HUVECcells using p42 MAPK antibody.
For research use only. Not for use in diagnostic procedures.