Product Name: ERK 1/2 Polyclonal Antibody
Catalog No.: ALT1625
Reactivity: Human;Mouse;Rat
Applications: IF/ICC;WB;IHC-p;ELISA,ChIP
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: IF: 1:50-200 Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: MAPK1/MAPK3
Protein Name: Mitogen-activated protein kinase 3
Human Gene ID: 5595
Human Swiss Prot No.: P27361/P28482
Other Name: MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK3; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p
Subcellular Location: Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .
Expression: Epithelium,Eye,Hepatoma,Human cervix,Lymph,
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p44/42 MAPK Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using p44/42 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.