Product Name: Ephrin-B1/2/3 (phospho Tyr324) Polyclonal Antibody
Catalog No.: ALP0812
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: EFNB1/EFNB2/EFNB3
Protein Name: Ephrin-B1/2/3
Human Gene ID: 1947/1948/1949
Human Swiss Prot No.: P98172/P52799/Q15768
Other Name: EFNB1; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB2; EPLG5; HTKL; LERK5; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L; EFNB3; EP
Subcellular Location: Cell membrane ; Single-pass type I membrane protein . Membrane raft . May recruit GRIP1 and GRIP2 to membrane raft domains. .; [Ephrin-B1 C-terminal fragment]: Cell membrane ; Single-pass type I membrane protein .; [Ephrin-B1 intracellular domain]: Nucleus . Colocalizes with ZHX2 in the nucleus. .
Expression: Widely expressed (PubMed:8070404, PubMed:7973638). Detected in both neuronal and non-neuronal tissues (PubMed:8070404, PubMed:7973638). Seems to have particulaYy strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638).
Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells treated with serum 20% 15′, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.