Product Name: EphA2/3/4 Polyclonal Antibody
Catalog No.: ALT1576
Reactivity: Human;Rat
Applications: WB;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: EPHA2/3/4
Protein Name: Ephrin type-A receptor 2/3/4
Human Gene ID: 1969/2042/2043
Human Swiss Prot No.: P29317/P29320/P54764
Other Name: EPHA2; ECK; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA3; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO
Subcellular Location: Cell membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane ; Single-pass type I membrane protein . Cell junction, focal adhesion . Present at regions of cell-cell contacts but also at the leading edge of migrating cells (PubMed:19573808, PubMed:20861311). Relocates from the plasma membrane to the cytoplasmic and perinuclear regions in cancer cells (PubMed:18794797). .
Expression: Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.
Immunofluorescence analysis of A549 cells, using EPHA2/3/4 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from MCF-7 cells, using EPHA2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.
For research use only. Not for use in diagnostic procedures.