Product Name: EMMPRIN Polyclonal Antibody

Catalog No.: ALT5471

Reactivity: Human;Rat;Mouse;

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: BSG

Protein Name: Basigin

Human Gene ID: 682

Human Swiss Prot No.: P35613

Other Name: BSG; Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147

Subcellular Location: Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .; [Isoform 1]: Cell membrane ; Single-pass type I membrane protein . Photoreceptor inner segment . Cell projection, cilium, photoreceptor outer segment .; [Isoform 2]: Cell membrane ; Single-pass type I membrane protein . Endosome . Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Basolateral cell membrane ; Single-pass type I membrane protein .; [Isoform 3]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 4]: Cell membrane ; Single-pass type I membrane protein .

Expression: [Isoform 1]: Retina-specific (PubMed:25957687). Expressed in retinal cone photoreceptors (at protein level) (PubMed:25957687). ; [Isoform 2]: Expressed in erythrocytes (at protein level) (PubMed:26195724, PubMed:28409866). Highly expressed in melanoma cell lines (at protein level) (PubMed:11992541). Highly expressed in the heart, kidney, skeletal muscle and testis (PubMed:21536654). ; [Isoform 3]: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus. ; [Isoform 4]: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus.

Western Blot analysis of HeLa cells using EMMPRIN Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

For research use only. Not for use in diagnostic procedures.

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