Product Name: E2F-1 Polyclonal Antibody
Catalog No.: ALT5811
Reactivity: Human;Mouse;Rat
Applications: IF/ICC;WB;IHC-p;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Storage Stability: -20°C/1 year
Gene Name: E2F1 RBBP3
Protein Name: E2F transcription factor 1
Human Gene ID: 1869
Human Swiss Prot No.: Q01094
Other Name: E2F1 RBBP3
Subcellular Location: Nucleus .
Expression: Brain,Epithelium,Pancreas,Skin,
Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,E2F-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of MOUSE-BRAIN cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
For research use only. Not for use in diagnostic procedures.