Product Name: Cortactin (phospho Tyr421) Polyclonal Antibody
Catalog No.: ALP0072
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: CTTN
Protein Name: Src substrate cortactin
Human Gene ID: 2017
Human Swiss Prot No.: Q14247
Other Name: CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1
Subcellular Location: Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle. Cell projection, dendrite . Cell projection . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, podosome . Cell junction . Cell junction, focal adhesion . Membrane, clathrin-coated pit . Cell projection, dendritic spine . Cytoplasm, cell cortex . Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity). .
Expression: Coronary artery,Epithelium,Mammary gland,Placenta,Platelet,Testis,
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cortactin (Phospho-Tyr421) Antibody
Western blot analysis of lysates from HeLa cells treated with H2O2, using Cortactin (Phospho-Tyr421) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.