Product Name: COL17A1 Polyclonal Antibody
Catalog No.: ALT5832
Reactivity: Human;Rat;Mouse;
Applications: WB IF;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: COL17A1 BP180 BPAG2
Protein Name: collagen, type XVII, alpha 1
Human Gene ID: 1308
Human Swiss Prot No.: Q9UMD9
Other Name: COL17A1 BP180 BPAG2
Subcellular Location: Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Localized along the plasma membrane of the hemidesmosome.; [120 kDa linear IgA disease antigen]: Secreted, extracellular space, extracellular matrix, basement membrane. Exclusively localized to anchoring filaments. Localized to the epidermal side of split skin.; [97 kDa linear IgA disease antigen]: Secreted, extracellular space, extracellular matrix, basement membrane. Localized in the lamina lucida beneath the hemidesmosomes.
Expression: Detected in skin (PubMed:8618013). In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200
Western Blot analysis of 3T3, hepg2 cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
For research use only. Not for use in diagnostic procedures.