Product Name: Chk1 (phospho Ser301) Polyclonal Antibody
Catalog No.: ALP0546
Reactivity: Human;Mouse;Rat
Applications: WB;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: CHEK1
Protein Name: Serine/threonine-protein kinase Chk1
Human Gene ID: 1111
Human Swiss Prot No.: O14757
Other Name: CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1
Subcellular Location: Nucleus . Chromosome . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063). .
Expression: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Chk1 (Phospho-Ser301) Antibody
Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells, using Chk1 (Phospho-Ser301) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.