Product Name: CENP-A (phospho Ser7) Polyclonal Antibody
Catalog No.: ALP1172
Reactivity: Human;Rat;Mouse;
Applications: IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Immunofluorescence: 1/200 – 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: CENPA
Protein Name: Histone H3-like centromeric protein A
Human Gene ID: 1058
Human Swiss Prot No.: P49450
Other Name: CENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A
Subcellular Location: Nucleus . Chromosome, centromere, kinetochore . Chromosome, centromere . Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during eaYy mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658). .
Expression: Epithelium,Uterus,
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Centromeric Protein A (Phospho-Ser7) Antibody
Immunofluorescence analysis of HeLa cells, using Centromeric Protein A (Phospho-Ser7) Antibody. The picture on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.