Product Name: Caspase-4 Polyclonal Antibody
Catalog No.: ALT0657
Reactivity: Human
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: CASP4
Protein Name: Caspase4
Human Gene ID: 837
Human Swiss Prot No.: P49662
Other Name: CASP4; ICH2; Caspase-4; CASP-4; ICE(rel)-II; Protease ICH-2; Protease TX
Subcellular Location: Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630). .
Expression: Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.
Immunohistochemistry analysis of Caspase-4 antibody in paraffin-embedded human lung carcinoma tissue.
Western blot analysis of lysate from K562 cells, using Caspase-4 antibody.
Immunohistochemistry analysis of Caspase-4 antibody in paraffin-embedded human lung carcinoma tissue.
Western blot analysis of lysates from 1) Hela ,2)THP-1, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour.
For research use only. Not for use in diagnostic procedures.