c-Src (phospho Tyr529) Polyclonal Antibody

Catalog No.: 

Product Name: c-Src (phospho Tyr529) Polyclonal Antibody

Catalog No.: ALP0078

Reactivity: Human;Mouse;Rat;Monkey

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/10000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: SRC

Protein Name: Proto-oncogene tyrosine-protein kinase Src

Human Gene ID: 6714

Human Swiss Prot No.: P12931

Other Name: SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Subcellular Location: Cell membrane ; Lipid-anchor . Mitochondrion inner membrane . Nucleus . Cytoplasm, cytoskeleton . Cytoplasm, perinuclear region . Cell junction, focal adhesion . Localizes to focal adhesion sites following integrin engagement (PubMed:22801373). Localization to focal adhesion sites requires myristoylation and the SH3 domain (PubMed:7525268). Colocalizes with PDLIM4 at the perinuclear region, but not at focal adhesions (PubMed:19307596). .

Expression: Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.; [Isoform 1]: Expressed in spleen and liver. ; [Isoform 2]: Expressed in brain. ; [Isoform 3]: Expressed in brain.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Src (Phospho-Tyr529) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (Phospho-Tyr529) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30′, using Src (Phospho-Tyr529) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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