Product Name: c-Kit (phospho Tyr703) Polyclonal Antibody
Catalog No.: ALP0658
Reactivity: Human;Mouse
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: KIT
Protein Name: Mast/stem cell growth factor receptor Kit
Human Gene ID: 3815
Human Swiss Prot No.: P10721
Other Name: KIT; SCFR; Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD antigen CD117
Subcellular Location: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cytoplasm . Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head. .
Expression: [Isoform 3]: In testis, detected in spermatogonia in the basal layer and in interstitial Leydig cells but not in Sertoli cells or spermatocytes inside the seminiferous tubules (at protein level) (PubMed:20601678). Expression is maintained in ejaculated spermatozoa (at protein level) (PubMed:20601678).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using KIT (Phospho-Tyr703) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30′, using KIT (Phospho-Tyr703) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.