Product Name: BUB1 Polyclonal Antibody
Catalog No.: ALT0546
Reactivity: Human;Rat;Mouse;
Applications: WB;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: BUB1
Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
Human Gene ID: 699
Human Swiss Prot No.: O43683
Other Name: BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A
Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very eaYy in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity). .
Expression: High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
Western blot analysis of the lysates from HUVECcells using BUB1 antibody.
For research use only. Not for use in diagnostic procedures.