Product Name: BAM32 (phospho Tyr139) Polyclonal Antibody
Catalog No.: ALP0549
Reactivity: Human;Mouse
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: DAPP1
Protein Name: Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Human Gene ID: 27071
Human Swiss Prot No.: Q9UN19
Other Name: DAPP1; BAM32; HSPC066; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; hDAPP1; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa
Subcellular Location: Cytoplasm . Membrane ; Peripheral membrane protein . Membrane-associated after cell stimulation leading to its translocation.
Expression: Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPP1 (Phospho-Tyr139) Antibody
Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2′, using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.