Product Name: Atg9a Polyclonal Antibody
Catalog No.: ALT5758
Reactivity: Human;Mouse;Rat
Applications: WB;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: WB 1:500-2000, ELISA 1:10000-20000
Storage Stability: -20°C/1 year
Gene Name: ATG9A APG9L1
Protein Name: Atg9a
Human Gene ID: 79065
Human Swiss Prot No.: Q7Z3C6
Other Name: ATG9 autophagy related 9 homolog A (S. cerevisiae)
Subcellular Location: Preautophagosomal structure membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Mitochondrion membrane ; Multi-pass membrane protein . Mainly localizes to the trans-Golgi network (TGN) and the endosomal system; cycles between them though vesicle trafficking (PubMed:27316455, PubMed:27663665). Export from the TGN to promote formation of autophagosomes is mediated by the AP-4 complex (PubMed:29180427, PubMed:30262884). Under amino acid starvation or rapamycin treatment, redistributes to preautophagosomal structure/phagophore assembly site (PAS) (PubMed:16940348). The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1 (PubMed:16940348). Upon autophagy induction, a small portion transiently localizes to the autophagic membranes (PubMed:22456507). Recruited to damaged mitochondria during mitophagy in a RIMOC1-dependent manner (PubMed:34432599). .
Expression: Brain,Embryo,Epithelium,Fetal brain,Lung endothelial cell,Lymph,Rectum tumor,Testis
Western Blot analysis of 293T 3T3 cells using Atg9a Polyclonal Antibody diluted at 1:800. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
For research use only. Not for use in diagnostic procedures.