APC (phospho Ser2054) Polyclonal Antibody

Catalog No.: 

Product Name: APC (phospho Ser2054) Polyclonal Antibody

Catalog No.: ALP0724

Reactivity: Human;Rat;Mouse;

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: APC

Protein Name: Adenomatous polyposis coli protein

Human Gene ID: 324

Human Swiss Prot No.: P25054

Other Name: APC; DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

Subcellular Location: Cell junction, adherens junction . Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle membrane . Cytoplasm . Cell membrane . Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment (PubMed:19151759). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854). .

Expression: Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver (PubMed:21643010, PubMed:27217144). Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues (PubMed:19527921, PubMed:21643010, PubMed:27217144). Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood (PubMed:19527921, PubMed:21643010, PubMed:27217144).

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using APC (Phospho-Ser2054) Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using APC (Phospho-Ser2054) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30′, using APC (Phospho-Ser2054) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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