Akt1 (phospho Tyr474) Polyclonal Antibody

Catalog No.: 

Product Name: Akt1 (phospho Tyr474) Polyclonal Antibody

Catalog No.: ALP0683

Reactivity: Human;Mouse;Rat;Monkey

Applications: WB;IHC-p;IF(paraffin section);ELISA

Source: Polyclonal, Rabbit,IgG

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications.

Storage Stability: -20°C/1 year

Gene Name: AKT1

Protein Name: RAC-alpha serine/threonine-protein kinase

Human Gene ID: 207

Human Swiss Prot No.: P31749

Other Name: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Subcellular Location: Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529). .

Expression: Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt (Phospho-Tyr474) Antibody


Immunohistochemistry analysis of paraffin-embedded human ovary, using Akt (Phospho-Tyr474) Antibody. The picture on the right is blocked with the phospho peptide.


Western blot analysis of lysates from COS7 cells treated with UV 15′, using Akt (Phospho-Tyr474) Antibody. The lane on the right is blocked with the phospho peptide.

For research use only. Not for use in diagnostic procedures.

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