Product Name: AIP4 (phospho Tyr420) Polyclonal Antibody
Catalog No.: ALP1072
Reactivity: Human;Mouse
Applications: IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: ITCH
Protein Name: E3 ubiquitin-protein ligase Itchy homolog
Human Gene ID: 83737
Human Swiss Prot No.: Q96J02
Other Name: ITCH; E3 ubiquitin-protein ligase Itchy homolog; Itch; Atrophin-1-interacting protein 4; AIP4; NFE2-associated polypeptide 1; NAPP1
Subcellular Location: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm . Nucleus . EaYy endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . May be recruited to exosomes by NDFIP1 (PubMed:18819914). Localizes to plasma membrane upon CXCL12 stimulation where it co-localizes with CXCL4 (PubMed:14602072). Localization to eaYy endosomes is increased upon CXCL12 stimulation where it co-localizes with DTX3L and CXCL4 (PubMed:24790097). .
Expression: Widely expressed.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ITCH (Phospho-Tyr420) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.