Product Name: Actin β Monoclonal Antibody
Catalog No.: ALM0012
Reactivity: Human;Mouse;Rat;Hamster;Monkey
Applications: WB;IF/ICC;FCM;ELISA
Source: Monoclonal, Mouse
Formulation: Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Concentration:
Dilution: Western Blot: 1/500 – 1/2000. Immunofluorescence: 1/200 – 1/1000. Flow cytometry: 1/200 – 1/400. ELISA: 1/10000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: ACTB
Protein Name: Actin cytoplasmic 1
Human Gene ID: 60
Human Swiss Prot No.: P60709
Other Name: ACTB; Actin; cytoplasmic 1; Beta-actin
Subcellular Location: Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Expression: B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
Western Blot analysis using Actin β Monoclonal Antibody against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Actin β Monoclonal Antibody (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of MCF-7 cells using Actin β Monoclonal Antibody (right) and negative control (left).
For research use only. Not for use in diagnostic procedures.