Product Name: AChRα1 Polyclonal Antibody
Catalog No.: ALT5555
Reactivity: Human;Mouse;Rat
Applications: WB;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: CHRNA1
Protein Name: Acetylcholine receptor subunit alpha
Human Gene ID: 1134
Human Swiss Prot No.: P02708
Other Name: CHRNA1; ACHRA; CHNRA; Acetylcholine receptor subunit alpha
Subcellular Location: Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein .
Expression: Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of NIH-3T3 cells using AChRα1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
For research use only. Not for use in diagnostic procedures.