Product Name: AAT Polyclonal Antibody
Catalog No.: ALT5056
Reactivity: Human;Rat;Mouse;
Applications: WB;IHC-p;IF(paraffin section);ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: SERPINA1
Protein Name: Alpha-1-antitrypsin
Human Gene ID: 5265
Human Swiss Prot No.: P01009
Other Name: SERPINA1; AAT; PI; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
Subcellular Location: Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellulaYy in the endoplasmic reticulum.; [Short peptide from AAT]: Secreted, extracellular space, extracellular matrix.
Expression: Ubiquitous. Expressed in leukocytes and plasma.
Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
For research use only. Not for use in diagnostic procedures.