Product Name: A-Raf (phospho Tyr302) Polyclonal Antibody
Catalog No.: ALP0904
Reactivity: Human;Mouse;Rat
Applications: WB;IHC-p;IF/ICC;ELISA
Source: Polyclonal, Rabbit,IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration:1 mg/ml
Dilution: Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Storage Stability: -20°C/1 year
Gene Name: ARAF
Protein Name: Serine/threonine-protein kinase A-Raf
Human Gene ID: 369
Human Swiss Prot No.: P10398
Other Name: ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
Subcellular Location: intracellular,mitochondrion,cytosol,
Expression: Predominantly in urogenital tissues.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using A-RAF (Phospho-Tyr302) Antibody
Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30′, using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide.
For research use only. Not for use in diagnostic procedures.